The cost-effective training solution, which will improve the safety of pilots, is being developed at RAF Cranwell.
The new training service is expected to create jobs and strengthen the UK supply chain. Thales will partner with many small and mid-sized enterprises (SMEs) during the construction phase of the service.
UK Defence Minister Philip Dunne said: "This £44m contract which will allow pilots to develop their ability to withstand high G-force in a safe environment, will involve the innovation and expertise of Small and Medium Enterprises across the UK.
"This is all part of our £178bn investment in giving our service men and women the very best possible training and equipment."
With Thales being the prime contractor in the deal, the company will be responsible for design, build and operation of the training service, which is planned to commence from 2018.
On the other hand, AMST will offer the design, technical and engineering capability for a human training centrifuge which allows pilots to build and maintain their ability to withstand high G in a safe environment.
AMST CEO Richard Schluesselberger said: "Together, AMST and Thales will provide the RAF with the optimum and above all, safest and most effective High G training."